Of both isotopes, with Δ18O alone accounting for ∼50%. Moreover, ∼80% of the variation in biomass across growing conditions and genotypes was explained by a combination However, genotypic differences in Δ13C were observed. Translated into significant differences in Δ18Os. Even though the four genotypes showed significant differences in cumulative transpiration rates and biomass, this was not Δ18O variation was mainly determined by the amount of transpired water throughout plant growth (Tcum), whereas Δ13C variation was explained in part by a combination of leaf N and stomatal conductance (gs). On the other hand, water supply decreased Δ18O values, whereas N did not affect this parameter. Conversely, N fertilizer significantly decreased Δ13C. In water supply did not lead to a significant decrease in gas exchange parameters, and consequently Δ13C was only slightly modified by water input. Water and N supply significantly increased plant growth. Here the combined effect of different water and N regimes on Δ13C, Δ18O, gas exchange, water-use efficiency (WUE), and growth of four genotypes of durum wheat cultured in pots was studied. Whereas the effects of water and nitrogen (N) on plant Δ13C have been reported previously, these factors have scarcely been studied for Δ18O.
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